diff --git a/web3.js/src/ed25519-program.ts b/web3.js/src/ed25519-program.ts
new file mode 100644
index 0000000000..fab806ea8a
--- /dev/null
+++ b/web3.js/src/ed25519-program.ts
@@ -0,0 +1,140 @@
+import {Buffer} from 'buffer';
+import * as BufferLayout from '@solana/buffer-layout';
+import nacl from 'tweetnacl';
+
+import {Keypair} from './keypair';
+import {PublicKey} from './publickey';
+import {TransactionInstruction} from './transaction';
+import assert from './util/assert';
+
+const PRIVATE_KEY_BYTES = 64;
+const PUBLIC_KEY_BYTES = 32;
+const SIGNATURE_BYTES = 64;
+
+/**
+ * Params for creating an ed25519 instruction using a public key
+ */
+export type CreateEd25519InstructionWithPublicKeyParams = {
+  publicKey: Uint8Array;
+  message: Uint8Array;
+  signature: Uint8Array;
+  instructionIndex?: number;
+};
+
+/**
+ * Params for creating an ed25519 instruction using a private key
+ */
+export type CreateEd25519InstructionWithPrivateKeyParams = {
+  privateKey: Uint8Array;
+  message: Uint8Array;
+  instructionIndex?: number;
+};
+
+const ED25519_INSTRUCTION_LAYOUT = BufferLayout.struct([
+  BufferLayout.u8('numSignatures'),
+  BufferLayout.u8('padding'),
+  BufferLayout.u16('signatureOffset'),
+  BufferLayout.u16('signatureInstructionIndex'),
+  BufferLayout.u16('publicKeyOffset'),
+  BufferLayout.u16('publicKeyInstructionIndex'),
+  BufferLayout.u16('messageDataOffset'),
+  BufferLayout.u16('messageDataSize'),
+  BufferLayout.u16('messageInstructionIndex'),
+]);
+
+export class Ed25519Program {
+  /**
+   * @internal
+   */
+  constructor() {}
+
+  /**
+   * Public key that identifies the ed25519 program
+   */
+  static programId: PublicKey = new PublicKey(
+    'Ed25519SigVerify111111111111111111111111111',
+  );
+
+  /**
+   * Create an ed25519 instruction with a public key and signature. The
+   * public key must be a buffer that is 32 bytes long, and the signature
+   * must be a buffer of 64 bytes.
+   */
+  static createInstructionWithPublicKey(
+    params: CreateEd25519InstructionWithPublicKeyParams,
+  ): TransactionInstruction {
+    const {publicKey, message, signature, instructionIndex} = params;
+
+    assert(
+      publicKey.length === PUBLIC_KEY_BYTES,
+      `Public Key must be ${PUBLIC_KEY_BYTES} bytes but received ${publicKey.length} bytes`,
+    );
+
+    assert(
+      signature.length === SIGNATURE_BYTES,
+      `Signature must be ${SIGNATURE_BYTES} bytes but received ${signature.length} bytes`,
+    );
+
+    const publicKeyOffset = ED25519_INSTRUCTION_LAYOUT.span;
+    const signatureOffset = publicKeyOffset + publicKey.length;
+    const messageDataOffset = signatureOffset + signature.length;
+    const numSignatures = 1;
+
+    const instructionData = Buffer.alloc(messageDataOffset + message.length);
+
+    ED25519_INSTRUCTION_LAYOUT.encode(
+      {
+        numSignatures,
+        padding: 0,
+        signatureOffset,
+        signatureInstructionIndex: instructionIndex,
+        publicKeyOffset,
+        publicKeyInstructionIndex: instructionIndex,
+        messageDataOffset,
+        messageDataSize: message.length,
+        messageInstructionIndex: instructionIndex,
+      },
+      instructionData,
+    );
+
+    instructionData.fill(publicKey, publicKeyOffset);
+    instructionData.fill(signature, signatureOffset);
+    instructionData.fill(message, messageDataOffset);
+
+    return new TransactionInstruction({
+      keys: [],
+      programId: Ed25519Program.programId,
+      data: instructionData,
+    });
+  }
+
+  /**
+   * Create an ed25519 instruction with a private key. The private key
+   * must be a buffer that is 64 bytes long.
+   */
+  static createInstructionWithPrivateKey(
+    params: CreateEd25519InstructionWithPrivateKeyParams,
+  ): TransactionInstruction {
+    const {privateKey, message, instructionIndex} = params;
+
+    assert(
+      privateKey.length === PRIVATE_KEY_BYTES,
+      `Private key must be ${PRIVATE_KEY_BYTES} bytes but received ${privateKey.length} bytes`,
+    );
+
+    try {
+      const keypair = Keypair.fromSecretKey(privateKey);
+      const publicKey = keypair.publicKey.toBytes();
+      const signature = nacl.sign.detached(message, keypair.secretKey);
+
+      return this.createInstructionWithPublicKey({
+        publicKey,
+        message,
+        signature,
+        instructionIndex,
+      });
+    } catch (error) {
+      throw new Error(`Error creating instruction; ${error}`);
+    }
+  }
+}
diff --git a/web3.js/src/index.ts b/web3.js/src/index.ts
index a7901a26b3..5b6a7f18d2 100644
--- a/web3.js/src/index.ts
+++ b/web3.js/src/index.ts
@@ -4,6 +4,7 @@ export * from './bpf-loader-deprecated';
 export * from './bpf-loader';
 export * from './connection';
 export * from './epoch-schedule';
+export * from './ed25519-program';
 export * from './fee-calculator';
 export * from './keypair';
 export * from './loader';
diff --git a/web3.js/test/ed25519-program.test.ts b/web3.js/test/ed25519-program.test.ts
new file mode 100644
index 0000000000..a2598f782b
--- /dev/null
+++ b/web3.js/test/ed25519-program.test.ts
@@ -0,0 +1,54 @@
+import {Buffer} from 'buffer';
+import nacl from 'tweetnacl';
+
+import {
+  Connection,
+  Keypair,
+  sendAndConfirmTransaction,
+  LAMPORTS_PER_SOL,
+  Transaction,
+  Ed25519Program,
+} from '../src';
+import {url} from './url';
+
+if (process.env.TEST_LIVE) {
+  describe('ed25519', () => {
+    const keypair = Keypair.generate();
+    const privateKey = keypair.secretKey;
+    const publicKey = keypair.publicKey.toBytes();
+    const from = Keypair.generate();
+    const connection = new Connection(url, 'confirmed');
+
+    before(async function () {
+      await connection.confirmTransaction(
+        await connection.requestAirdrop(from.publicKey, 10 * LAMPORTS_PER_SOL),
+      );
+    });
+
+    it('create ed25519 instruction', async () => {
+      const message = Buffer.from('string address');
+      const signature = nacl.sign.detached(message, privateKey);
+      const transaction = new Transaction().add(
+        Ed25519Program.createInstructionWithPublicKey({
+          publicKey,
+          message,
+          signature,
+        }),
+      );
+
+      await sendAndConfirmTransaction(connection, transaction, [from]);
+    });
+
+    it('create ed25519 instruction with private key', async () => {
+      const message = Buffer.from('private key');
+      const transaction = new Transaction().add(
+        Ed25519Program.createInstructionWithPrivateKey({
+          privateKey,
+          message,
+        }),
+      );
+
+      await sendAndConfirmTransaction(connection, transaction, [from]);
+    });
+  });
+}