diff --git a/os/hal/ports/STM32/LLD/DMAv1/stm32_dma.h b/os/hal/ports/STM32/LLD/DMAv1/stm32_dma.h
index 54b6bde84..1a6c98691 100644
--- a/os/hal/ports/STM32/LLD/DMAv1/stm32_dma.h
+++ b/os/hal/ports/STM32/LLD/DMAv1/stm32_dma.h
@@ -38,6 +38,11 @@
  */
 #define STM32_DMA_ADVANCED          FALSE
 
+/**
+ * @brief   Maximum number of transfers in a single operation.
+ */
+#define STM32_DMA_MAX_TRANSFER      65535
+
 /**
  * @brief   Total number of DMA streams.
  * @details This is the total number of streams among all the DMA units.
diff --git a/os/hal/ports/STM32/LLD/DMAv2/stm32_dma.h b/os/hal/ports/STM32/LLD/DMAv2/stm32_dma.h
index 256203932..9a6584541 100644
--- a/os/hal/ports/STM32/LLD/DMAv2/stm32_dma.h
+++ b/os/hal/ports/STM32/LLD/DMAv2/stm32_dma.h
@@ -36,6 +36,11 @@
  */
 #define STM32_DMA_ADVANCED          TRUE
 
+/**
+ * @brief   Maximum number of transfers in a single operation.
+ */
+#define STM32_DMA_MAX_TRANSFER      65535
+
 /**
  * @brief   Total number of DMA streams.
  * @details This is the total number of streams among all the DMA units.
diff --git a/os/hal/ports/STM32/LLD/SPIv1/hal_spi_lld.c b/os/hal/ports/STM32/LLD/SPIv1/hal_spi_lld.c
index 0f5d6904e..9470e4c24 100644
--- a/os/hal/ports/STM32/LLD/SPIv1/hal_spi_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv1/hal_spi_lld.c
@@ -534,7 +534,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 void spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &dummyrx);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -566,7 +566,7 @@ void spi_lld_ignore(SPIDriver *spip, size_t n) {
 void spi_lld_exchange(SPIDriver *spip, size_t n,
                       const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -595,7 +595,7 @@ void spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &dummyrx);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -624,7 +624,7 @@ void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 void spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
diff --git a/os/hal/ports/STM32/LLD/SPIv1/hal_spi_v2_lld.c b/os/hal/ports/STM32/LLD/SPIv1/hal_spi_v2_lld.c
index 544850fa0..20f6e28bb 100644
--- a/os/hal/ports/STM32/LLD/SPIv1/hal_spi_v2_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv1/hal_spi_v2_lld.c
@@ -720,7 +720,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &spip->rxsink);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -755,7 +755,7 @@ msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
                        const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -787,7 +787,7 @@ msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &spip->rxsink);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -819,7 +819,7 @@ msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 msg_t spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
diff --git a/os/hal/ports/STM32/LLD/SPIv2/hal_spi_lld.c b/os/hal/ports/STM32/LLD/SPIv2/hal_spi_lld.c
index 69d54d99e..f20e24352 100644
--- a/os/hal/ports/STM32/LLD/SPIv2/hal_spi_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv2/hal_spi_lld.c
@@ -559,7 +559,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 void spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &dummyrx);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -591,7 +591,7 @@ void spi_lld_ignore(SPIDriver *spip, size_t n) {
 void spi_lld_exchange(SPIDriver *spip, size_t n,
                       const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -620,7 +620,7 @@ void spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &dummyrx);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -649,7 +649,7 @@ void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 void spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
diff --git a/os/hal/ports/STM32/LLD/SPIv2/hal_spi_v2_lld.c b/os/hal/ports/STM32/LLD/SPIv2/hal_spi_v2_lld.c
index 1ecfe2fc0..ef6787c99 100644
--- a/os/hal/ports/STM32/LLD/SPIv2/hal_spi_v2_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv2/hal_spi_v2_lld.c
@@ -730,7 +730,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &spip->rxsink);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -765,7 +765,7 @@ msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
                        const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -797,7 +797,7 @@ msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, &spip->rxsink);
   dmaStreamSetTransactionSize(spip->dmarx, n);
@@ -829,7 +829,7 @@ msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 msg_t spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   dmaStreamSetMemory0(spip->dmarx, rxbuf);
   dmaStreamSetTransactionSize(spip->dmarx, n);
diff --git a/os/hal/ports/STM32/LLD/SPIv3/hal_spi_lld.c b/os/hal/ports/STM32/LLD/SPIv3/hal_spi_lld.c
index cf1995cf8..96b84ac05 100644
--- a/os/hal/ports/STM32/LLD/SPIv3/hal_spi_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv3/hal_spi_lld.c
@@ -825,7 +825,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 void spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   spi_lld_wait_complete(spip);
 
@@ -885,7 +885,7 @@ void spi_lld_ignore(SPIDriver *spip, size_t n) {
 void spi_lld_exchange(SPIDriver *spip, size_t n,
                       const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   spi_lld_wait_complete(spip);
 
@@ -942,7 +942,7 @@ void spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   spi_lld_wait_complete(spip);
 
@@ -999,7 +999,7 @@ void spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 void spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
   spi_lld_wait_complete(spip);
 
diff --git a/os/hal/ports/STM32/LLD/SPIv3/hal_spi_v2_lld.c b/os/hal/ports/STM32/LLD/SPIv3/hal_spi_v2_lld.c
index 8ffb15040..2ec431edd 100644
--- a/os/hal/ports/STM32/LLD/SPIv3/hal_spi_v2_lld.c
+++ b/os/hal/ports/STM32/LLD/SPIv3/hal_spi_v2_lld.c
@@ -1069,7 +1069,7 @@ void spi_lld_unselect(SPIDriver *spip) {
  */
 msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
 #if defined(STM32_SPI_DMA_REQUIRED) && defined(STM32_SPI_BDMA_REQUIRED)
   if (spip->is_bdma)
@@ -1130,7 +1130,7 @@ msg_t spi_lld_ignore(SPIDriver *spip, size_t n) {
 msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
                        const void *txbuf, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
 #if defined(STM32_SPI_DMA_REQUIRED) && defined(STM32_SPI_BDMA_REQUIRED)
   if (spip->is_bdma)
@@ -1188,7 +1188,7 @@ msg_t spi_lld_exchange(SPIDriver *spip, size_t n,
  */
 msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
 #if defined(STM32_SPI_DMA_REQUIRED) && defined(STM32_SPI_BDMA_REQUIRED)
   if (spip->is_bdma)
@@ -1246,7 +1246,7 @@ msg_t spi_lld_send(SPIDriver *spip, size_t n, const void *txbuf) {
  */
 msg_t spi_lld_receive(SPIDriver *spip, size_t n, void *rxbuf) {
 
-  osalDbgAssert(n < 65536, "unsupported DMA transfer size");
+  osalDbgAssert(n <= STM32_DMA_MAX_TRANSFER, "unsupported DMA transfer size");
 
 #if defined(STM32_SPI_DMA_REQUIRED) && defined(STM32_SPI_BDMA_REQUIRED)
   if (spip->is_bdma)
diff --git a/readme.txt b/readme.txt
index c755f58d9..7806c8a50 100644
--- a/readme.txt
+++ b/readme.txt
@@ -74,6 +74,7 @@
 *****************************************************************************
 
 *** Next ***
+- NEW: STM32 DMA drivers now export an STM32_DMA_MAX_TRANSFER definition.
 - NEW: PAL events for RP2040 added.
 - NEW: Added a "waend" field to the thread structure in RT for debug
        convenience.